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University of Connecticut Health Center - Know Better Care The Gregory P. Mullen NMR Structural Biology Facility & Biophysical Core Facility

Fluorescence Spectroscopy

CD

Hitachi F-2500 Fluorescence Spectrophotometer

This is a walk-up instrument or samples can be dropped off to be run for an additional fee. Training on the instrument is also available. Scheduling of this instrument is done through the FACES (http://faces.ccrc.uga.edu/) scheduling system. See the Calendars and Scheduling page for more information. Visit the Research Store for current pricing.

Proteins with TRP residues, will emit light at a longer wavelength (fluorescence) after they have absorbed light. The wavelength at which the maximum fluorescence occurs is dependent on the environment of the florophore and thus fluorescence spectroscopy is a useful technique to study structural changes of proteins due to conditions or through interactions with ligands. Typically protein concentrations in the 1 - 10 uM range with a 1 ml sample volume are needed. Scattering of light from dust or other precipitates is problematic so filtering samples is often desirable.